Renewable Column for Electrospray - MS
3.2.12.
Automated capture and release of biotin-containing conjugates on immobilized Streptavidin is a novel approach to automatedsample pretreatment at the front end to electrospray ionization mass spectrometry (ESI-MS).
Automated capture and dissociation rate constants for release from the Streptavidin of two chromophore-tagged biotin conjugates (BSN- Biotin-Sarcosin-PEG-Nitrobenzene and BPN- Biotin-PEG-Nitrobenzene) were measured by UV/VIS spectroscopy on the bead column within the LOV module, while the dissociation was simultaneously monitored by ESI-MS. The LOV-ESI-MS instrument was also used for repetitive assays of lysosomal a-galactosidase in human cell homogenates. Rapid assay turnover in 4.5 min/cycle and robust operation in 60 repetitive analyses were achieved, thus making LOV-ESI-MS technology suited for clinical practice.
